基本信息:

 姓   名:张静柏  性别:女   民族:汉 族   学位:博士  职称:讲师 
 所属单位:华中农业大学植物科学技术学院
 研究方向:植物与镰刀菌分子互作、植物抗病基因工程
 联系方式:jingbozhang@mail.hzau.edu.cn
 


 
    张静柏,女,博士,华中农业大学植物科学技术学院教师。1998-2002年就读于于河北农业大学植物保护专业,2002-2008年就读于华中农业大学攻读植物病理学专业博士学位,2008年1月获农学博士学位,毕业留校任教,承担《农业植物病理学实验》、《植物病害流行学》、《园艺植物病理学》、《园林植物病理学》等课程教学。从研究生阶段起至今,一直从事镰刀菌分子生物学、镰刀菌及其毒素特异抗体分离与毒素分析检测、镰刀菌与植物互作分子生物学、植物抗病及病菌致病相关基因克隆鉴定等方面研究,主持相关国家自然基金青年科学基金、教育部高等学校博士学科点专项新教师基金、武汉市科技攻关计划项目各1项,曾作为主要参与者完成国家“863”、“973”、“中德国际合作重点项目”多个国家课题,现作为骨干力量参加国家重大科研专项-转基因新品种培育1项、国家自然基金面上项目1项,共计发表研究论文30余篇(SCI论文32篇),授权或申请专利15项,其中以第一作者(共同第一作者)或通讯作者发表论文8篇, SCI收录6篇。
科研项目
1.国家自然科学基金青年科学基金项目:“赤霉菌致病相关基因CR1 的鉴定和功能分析”(2013-2015,主持)
2.教育部新教师基金:“我国长江中下游小麦、玉米赤霉菌系统群及产毒性比较研究”(2009-2012,主持)
3.武汉市科技计划项目:“抗伏马菌素特异重组抗体的分离及应用”(2013-2014,主持)
4.国家科技重大专项转基因生物新品种培育项目:“高抗赤霉病和毒素转基因小麦新品种培育”(2014-2016,参加)
5.农业部重大专项:“抗赤霉病转基因小麦新品系和新种质创制”(2011-2015,参加)
6.国家自然科学基金面上项目:“抗病抗体识别的赤霉病菌表面抗原基因克隆与功能鉴定”(2013-2016,参加)
发明专利
授权专利
1.抗伏马菌素B1的单克隆重组抗体H2. 廖玉才,李和平,胡祖权,张静柏. 专利号号:ZL 01310211887.4.
2.抗伏马菌素B1的单克隆重组抗体H7. 廖玉才,李和平,胡祖权,张静柏. 专利号号:ZL 201310214201.7.
3.镰刀菌特异单链抗体及制备方法和应用. 廖玉才,胡祖权,李和平,张静柏.专利号:ZL 201210574749.8.
4.一种镰刀菌单端孢霉烯族A类毒素的分子鉴定方法. 廖玉才,王建华,李和平,张静柏,黄涛.专利号:ZL 201010506095.6
5.适合转基因小麦定量检测的内标准基因引物设计及应用. 廖玉才, 李和平,赵正喜,瞿波,黄涛,张静柏等. 专利号:ZL 201110432297.5.
6.玉米赤霉烯酮毒素的分子检测方法及应用. 廖玉才,王建华,李和平,张静柏等. 专利号:ZL 201010511771.9
7.一种高亲和力镰刀菌特异单链抗体及其制备方法. 廖玉才,刘锦龙,李和平,张静柏. 专利号:ZL 201110407999.8.
8.抗镰刀菌单链抗体的筛选及应用. 廖玉才,李和平,胡祖权,张静柏. 专利号:ZL 201010527093.5
9.抗伏马菌素单链抗体的筛选及应用. 廖玉才,李和平,胡祖权,张静柏. 专利号:ZL 201010527116.2
10.一种镰刀菌单端孢霉烯族B类毒素的分子鉴定方法. 廖玉才,王建华,李和平,张静柏等. 专利号:ZL 201010506091.8
11.一种小麦赤霉病穗腐和苗腐抗性的分子标记.廖玉才,李和平,李旭,瞿波,张静柏. 专利号:ZL 201010028986.5.

已申请的专利
1.一种禾谷镰刀菌葡聚糖合成酶基因GLS及应用.廖玉才,李和平,张静柏等. 申请号:201510823894.9
2.防治作物储藏期黄曲霉菌及毒素的海藻希瓦氏菌及其应用. 廖玉才,宫安东,李和平,张静柏.申请号:201510133782.0
3.一株防治小麦赤霉病的解淀粉芽孢杆菌及其应用. 廖玉才,宫安东,李和平,张静柏. 申请号:201410321676.0

 发表的论文

第一作者或共同第一作者或通讯作者文章:
1. Hu, Z.-Q., Li, H.-P., Liu, J.-L., Xue, S., Gong, A.-D., Zhang, J.-B*., Liao, Y.-C. Production of a phage-displayed mouse scFv antibody against Fumonisin B1 and molecular docking analysis of their interactions. Biotechnology and Bioprocess Engineering, 2016, 2016, 21: 134-143.
2. Wang, J.-H., Zhang, J.-B.#, Li, H.-P., Gong, A.-D., Liao, Y.-C. Molecular identification, mycotoxin production and comparative pathogenicity of Fusarium temperatum isolated from maize in China. Journal of Phytopathology, 2014, 162: 147-157.
3. Zhang, J.-B., Wang, J.-H., Gong, A.-D., Chen, F.-F., Song, B., Li, H.-P., Li, X., Peng, C.-H., Liao Y.-C*. Natural occurrence of Fusarium head blight, mycotoxins, and mycotoxin-producing strains of Fusarium in commercial fields of wheat in Hubei. Plant Pathology, 2013, 62: 92-102.
4. Li, X., Zhang, J.-B.#, Song, B., Li, H.-P., Xu, H. Q., Qu, B., Dang, F.-J., Liao, Y.-C*. Resistance to Fusarium head blight and seedling blight in wheat is associated with activation of a cytochrome P450 gene. Phytopathology, 2010, 100:183-191.
5. Li, H.-P., Zhang, J.-B.#, Shi, R.-P., Huang, T., Fischer, R., Liao, Y.-C. Engineering Fusarium head blight resistance in wheat by expression of a fusion protein containing a Fusarium-specific antibody and an antifungal peptide. Molecular Plant-Microbe Interaction, 2008, 21:1242-1248.
6. Zhang, J.-B., Li, H.-P., Dang, F.-J., Qu, B., Xu, Y.-B., Zhao, C.-S., Liao, Y.-C. Determination of the trichothecene mycotoxin chemotypes and associated geographical distribution and phylogenetic species of the Fusarium graminearum clade from China. Mycological Research, 2007, 111:967-975.
其他作者文章:
1. Song, X.-S., Xing, S., Li, H.-P., Zhang, J.-B., Qu, B., Jiang, J.-H., Fan, C., Yang, P., Liu, J.-L., Hu, Z.-Q., Xue, S., Liao, Y.-C*. An antibody that confers plant disease resistance targets a membrane-bound glyoxal oxidase in Fusarium. New Phytologist, 2015, doi: 10.1111/nph.13806.
2. Cheng, W., Song, X.-S., Li, H.-P., Cao, L.-H., Sun, K., Qiu, X.-L., Xu, Y.-B., Yang, P., Huang, T., Zhang, J.-B., Qu, B., Liao, Y.-C. Host-induced gene silencing of an essential chitin synthase gene confers durable resistance to Fusarium head blight and seedling blight in wheat. Plant Biotechnology Journal, 2015, 13, 1335-1345.
3. Cheng, W., Li, H.-P., Zhang, J.-B., Du, H.-J., Wei, Q.-Y., Huang, T., Yang, P., Kong, X.-W., Liao, Y.-C. Tissue-specific and pathogen-inducible expression of a fusion protein containing a Fusarium-specific antibody and a fungal chitinase protects wheat against Fusarium pathogens and mycotoxins. Plant Biotechnology Journal, 2015, 13: 664-674.
4. Gong, A,-D., Li, H.-P., Shen, L., Zhang, J.-B., Wu, A.-B., He, W.-J., Yuan, Q.-S., He, J.-D., Liao, Y.-C. The Shewanella algae strain YM8 produces volatiles with strong inhibition activity against Aspergillus pathogens and aflatoxins. Frontiers in Microbiology, 2015, Oct 6. doi: 10.3389/fmicb.2015.01091.
5. Hu, Z.-Q., Li, H.-P., Wu, P., Li, Y.-B., Zhou, Z.-Q., Zhang, J.-B., Liu, J.-L., Liao, Y.-C*. An affinity improved single-chain antibody from phage display of a library derived from monoclonal antibodies detects fumonisins by immunoassay. Analytica Chimica Acta, 2015, 867: 74-82.
6. Gong, A.-D., Li, H.-P., Yuan, Q.-S., Song, X.-S., Yao, W., He, W.-J., Zhang, J.-B., Liao, Y.-C. Antagonistic mechanism of iturin A and plipastatin A from Bacillus amyloliquefaciens S76-3 from wheat spikes against Fusarium graminearum. PLoS ONE, 2015 Feb 17;10(2):e0116871. doi: 10.1371/journal.pone.0116871.
7. Song, X.-S., Li, H.-P., Zhang, J.-B., Song, B., Huang, T., Du, X.-M., Gong, A.-D., Liu, Y.-K., Feng, Y.-N. Agboola, R.S., Liao, Y.-C. Trehalose 6-phosphate phosphatase is required for development, virulence and mycotoxin biosynthesis apart from trehalose biosynthesis in Fusarium graminearum. Fungal Genetics and Biology, 2014, 63: 24-41.
8. Wang, J.-H., Li, H.-P., Zhang, J.-B., Wang, B.-T., Liao, Y.-C. First report of Fusarium maize ear rot caused by Fusarium kyushuense in China. Plant Disease, 2014, 98: 279-280. DOI: 10.1094/PDIS-05-13-0558-PDN.
9. Xue, S., Li, H.-P., Zhang, J.-B., Liu, J.-L., Hu, Z.-Q., Gong, A.-D., Huang, T., Liao, Y.-C. Chicken single-chain antibody fused to alkaline phosphatase detects Aspergillus pathogens and their presence in natural samples by direct sandwich enzyme-linked immunosorbent assay. Analytical Chemistry, 2013, 85, 10992–10999.
10. Song, B., Li, H.-P., Zhang, J.-B., Wang, J.-H., Gong, A.-D., Song, X.-S., Chen T., Liao, Y.-C. Type II myosin gene in Fusarium graminearum is required for septation, development, mycotoxin biosynthesis and pathogenicity. Fungal Genetics and Biology, 2013, 54: 60-70.
11. Hu, Z.-Q., Li, H.-P., Zhang, J.-B., Huang, T., Liu, J.-L., Xue, S., Wu, A.-B., Liao, Y.-C. A phage-displayed chicken single-chain antibody fused to alkaline phosphatase detects Fusarium pathogens and their presence in cereal grains. Analytica Chimica Acta, 2013, 764: 84-92.
12. Gao, C.-S., Kou, X.-J., Li, H.-P., Zhang, J.-B., Saad, A. Sefyan I., Liao, Y.-C. Inverse effects of Arabidopsis NPR1 gene on fusarium seedling blight and fusarium head blight in transgenic wheat. Plant Pathology, 2013, 62: 383-392.
13. Liu, Z.-W., Li, H.-P., Cheng, W., Yang, P., Zhang, J.-B., Gong, A.-D., Feng, Y.-N., Fernando, W. G. D., Liao, Y.-C. Enhanced overall resistance to Fusarium seedling blight and Fusarium head blight in transgenic wheat by co-expression of anti-fungal peptides. European Journal of Plant Pathology, 2012, 134: 721-732.
14. Hu, Z.-Q., Liu, J. L., Li, H.-P., Xing, S., Xue, S., Zhang, J.-B., Wang, J.-H., Nölke, G., Liao, Y.-C. Generation of a highly reactive chicken-derived single-chain variable fragment against Fusarium verticillioides by phage display. International Journal of Molecular Sciences, 2012, 13, 7038-7056.
15. Ndoye, M., Zhang, J-B., Wang, J.-H., Gong, A.-D, Qu, B., Li, H.-P., Li, S.-J, Liao, Y.-C. Nivalenol and 15-acetyldeoxynivalenol chemotypes of Fusarium graminearum clade species are prevalent on maize throughout China. Journal of Phytopathology, 2012, 160: 519-524.
16. Wang, J.-H., Zhang, J-B., Chen, F.- F., Li, H.-P., Ndoye M., Liao, Y.-C. A multiplex PCR assay for genetic chemotyping of toxigenic Fusarium graminearum and wheat grains for 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol and nivalenol mycotoxins. Journal of Food, Agriculture and Environment. 2012, 10: 505-511.
17. Liu, J.-L., Hu, Z.-Q., Xing, S., Xue, S., Li, H.-P., Zhang, J.-B., Liao, Y.-C. Attainment of 15-fold higher affinity of a Fusarium-specific single-chain antibody by directed molecular evolution coupled to phage display. Molecular Biotechnology, 2012, 52: 111-122.
18. Liu, G., Han, Z., Nie, D., Yang, J., Zhao, Z. H., Zhang, J. B., Li, H.P., Liao, Y. C., Song, S., Saeger, S. D., Wu, A. B. Rapid and sensitive quantitation of zearalenone in food and feed by lateral flow immunoassay. Food Control, 2012, 27: 200-205.
19. Han, Z., Zhao, Z.-Y., Song, S.-Q., Liu, G., Shi, J.-X., Zhang, J.-B., Liao, Y.-C., Zhang, D.-B., Saeger, S. D., Wu, A.-B. Establishment of a model isotope dilution LC-MS/MS method revealing kinetics and distribution of co-occurring mycotoxins in rat. Analytical Methods, 2012, 4, 3708–3717.
20. Wang, J.-H., Ndoye, M., Zhang, J.-B., Li, H.-P., Liao, Y.-C. Population structure and genetic diversity of the Fusarium graminearum species complex. Toxins, 2011, 3, 1020-1037.
21. Xu, Y.-B., Li, H.-P., Zhang, J.-B., Song, B., Chen, F.-F., Duan, X.-J., Xu, H.-Q., Liao, Y.-C. Disruption of the chitin synthase gene Chs1 from Fusarium asiaticum results in an altered structure of cell walls and reduced virulence. Fungal Genetics and Biology, 2010, 47: 205-215.
22. Wang, J.-H., Li, H.-P., Qu, B., Zhang, J.-B., Huang, T., Chen, F.-F., Liao, Y.-C. Development of a generic PCR detection of 3-acetyldeoxynivalenol-, 15-acetyldeoxynivalenol- and nivalenol-chemotypes of Fusarium graminearum clade. International Journal of Molecular Sciences, 2008, 9:2495-2504.
23. Hu, Z.-Q., Li, H.-P., Zhang, J.-B., Glinka, E., Liao, Y.-C. Antibody-mediated prevention of Fusarium mycotoxins in the field. International Journal of Molecular Sciences, 2008, 9: 1915-1926.
24. Qu, B., Li, H.-P., Zhang, J.-B., Xu, Y.-B., Huang, T., Wu, A.B., Zhao, C.-S., Carter, J., Nicholson, P., Liao, Y.-C. Geographical distribution and genetic diversity of the Fusarium graminearum and F. asiaticum on wheat spikes throughout China. Plant Pathology, 2008, 57: 15-24.
25. Qu, B., Li, H.-P., Zhang, J.-B., Huang, T., Carter, J., Liao, Y.-C., P. Nicholson. Comparison of genetic diversity and pathogenicity of Fusarium head blight pathogens from China and Europe revealed by SSCP and seedling assays on wheat. Plant Pathology, 2008, 57:642-651.
26. Liao, Y.-C., Li, H.-P., Yao, M.-J., Zhang, J.-B., Liu, J.-L. Plantibodies: A novel strategy to create pathogen-resistant plants. Biotechnology and Genetic Engineering Reviews, 2006, 23:253-271.
中文文章:
1. 胡祖权,李和平,刘锦龙,吴平,廖玉才,张静柏*. 抗伏马菌素B1单克隆重组抗体的筛选. 基因组学与应用生物学,2015,34(9):1912-1916
2. 胡祖权,李和平,吴平,廖玉才,张静柏*. 抗镰刀菌单链抗体在大肠杆菌中可溶性表达条件的研究. 生物技术通报,2015,31(9):238-243
3. 胡祖权,李和平,张静柏,刘锦龙,廖玉才. 伏马菌素B1特异单链抗体的同源建模及分子对接模拟研究. 免疫学杂志,2015,31(7):618-622
4. 李和平,张静柏,廖玉才. 绿色荧光蛋白基因在小麦禾谷镰刀菌中的表达与鉴定. 麦类作物学报,2010,30(5): 824-828
5. 刘春雷,廖玉才,张静柏,黄涛,李和平. 赤霉病菌特异抗体-防御素融合蛋白的表达和功能鉴定. 华中农业大学学报,2007,26(4):435-438
6. 武爱波,李和平,张静柏,赵纯森,姚明镜,SCHOLTEN Olga,廖玉才. 中国与欧洲禾谷镰刀菌DON毒素HPLC定量比较分析. 应用与环境生物学报,2007,13(1):131-134
7. 武爱波,李和平,张静柏,姚明镜,许玉彬,廖玉才. 小麦和玉米籽粒赤霉菌产毒类型的PCR检测. 麦类作物学报,2005,25( 5):1-4