研究成果-麦类作物分子生物技术实验室

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实验室近期成果

一、获得及申请的专利：

1．Fischer, R., Schillberg, S., Naehring, J., Sack, M., Monecke, M., Liao, Y.-C., Spiegel, H., Zimmerman, S., Emans, N. &Holzem, A. Molecular
      pathogenicide mediated plant disease resistance. US Patent Number 6825325 B1, Issued on Nov. 30, 2004.

2．Fischer, R., Schillberg, S., Naehring, J., Sack, M., Monecke, M., Liao, Y.-C., Spiegel, H., Zimmerman, S., Emans, N. &Holzem, A. Molecular
      pathogenicide mediated plant disease resistance. Indian Patent Number 192141, Issued on Aug. 17，2004.

3. 廖玉才，武爱波，李和平，赵纯森. 镰刀菌毒素的分子检测方法及应用. 专利号：ZL 200410009300.2; 授权日：2007年4月
   25日.

4. Peschen, D., Fischer, R. ，Liao, Y.-C.， Schillberg, S. & Dorfmueller, S. Antibodies,recombinant antibodies, recombinant antibody
      fragments and fusions mediated plant disease resistance against fungi. PCT/EP03/03852, 2002.

5. 廖玉才，李和平，黄涛一种非抗生素筛选小麦转基因植物的方法申请号：2010101400145400 专利申请人：华中农业大学

6. 廖玉才，李和平，黄涛，王韬. 一种农杆菌介导的无抗生素筛选大麦转基因植株的方法. 专利号：ZL 2007 1 0051390.5

申请号： 200710051390.5. 专利申请日：2007年01月26日专利申请人：华中农业大学授权公告日：2010年05月19日

7. 廖玉才，李和平，刘春雷，黄涛. 一种DNA的多点定向突变方法. 申请号：200710052273.0.

二、近期发表的部分论文（^* 通讯作者）：

1。Wang, J.-H., Li, H.-P., Qu, B., Zhang, J.-B., Huang, T., Chen, F.-F., Liao, Y.-C^*.Development of a generic PCR detection of 3-acetyldeoxynivalenol-, 15-acetyldeoxynivalenol- and nivalenol-chemotypes of Fusarium graminearum clade. International Journal of Molecular Sciences, 2008, 9, in press.

2。 Li, H.-P., Zhang, J.-B., Qu, B., Huang, T., Chen, F.-F., Zhao, C-S., Xu, Y.-B., Wu, A.-B., P. Nicholson, Liao, Y.-C^*. Phylogenetic species, mycotoxin chemotypes and geographical distribution of Fusarium asiaticum and F. graminearum on wheat spikes throughout China. Journal of Plant Pathology, 2008, 90:3-S3, 89.

3。 Li, H.-P., Zhang, J.-B., Shi, R.-P., Huang, T., Fischer, R., Liao, Y.-C^*. Engineering Fusarium head blight resistance in wheat by expression of a fusion protein containing a Fusarium-specific antibody and an antifungal peptide. Molecular Plant-Microbe Interaction, 2008, 21:1242-1248.

4。 Hu, Z.-Q., Li, H.-P., Zhang, J.-B., Glinka, E., Liao, Y.-C^*.Antibody-mediated prevention of Fusarium mycotoxins in the field. International Journal of Molecular Sciences, 2008, 9 (10): 1915-1926.

5。Li, H.-P., Huang, T., Wang, C.-X., Liao, Y.-C^*.A highly efficient regeneration system from leaf base segments of commercial cultivars of barley (Hordeum vulgare L.).Biologia Plantarum, 2008, in press.

6。 Qu, B., Li, H.-P., Zhang, J.-B., Xu, Y.-B., Huang, T., Wu, A.B.,Zhao, C.-S.,Carter, J., Nicholson, P., Liao, Y.-C^*. Geographical distribution and genetic diversity of the Fusarium graminearum and F. asiaticum on wheat spikes throughout China. Plant Pathology, 2008, 57: 15-24.

7。Qu, B., Li, H.-P., Zhang, J.-B., Huang, T., Carter, J., Liao, Y.-C^*., P. Nicholson. Comparison of genetic diversity and pathogenicity of Fusarium head blight pathogens from China and Europe revealed by SSCP and seedling assays on wheat.Plant Pathology, 2008, 57:642-651.

8。Zhang, J.-B., Li, H.-P., Dang, F.-J., Qu, B., Xu, Y.-B., Zhao, C.-S., Liao, Y.-C^*. Determination of the trichothecene mycotoxin chemotypes and associated geographical distribution and phylogenetic species of theFusarium graminearum clade from China. Mycological Research, 2007, 111:967-975.

9。Liao, Y.-C^*., Li, H.P., Liu W., Xing, S., Liu, J.L., Zhang, J.B. Generation of recombinant antibodies specific for Sclerotinia sclerotiorum.Proceedings of the 12th International Rapeseed Congress. Volume II，p162-165, March 26^th to 31^st, 2007, Wuhan, China.

10。 Liao, Y.-C^*., Li, H.-P., Zhao, C.-S., Yao, M.-J., Zhang, J.-B., Liu, J.-L. Plantibodies: A novel strategy to create pathogen-resistant plants. Biotechnology and Genetic Engineering Reviews, 2006, 23:253-271.

11。 Li, H.-P., Wu, A.-B., Zhao, C.-S., Scholten, O., Loeffler, H., Liao, Y.-C^*.Development of a generic PCR detection of deoxynivalenol- and nivalenol-chemotypes of Fusarium graminearum. FEMS Microbiology Letters, 2005, 243:505-511.

12。 Wu, A.-B.，Li., H.-P., Zhao, C.-S., Liao, Y.-C^*. Comparative pathogenicity of Fusarium graminearum from China revealed by wheat coleoptile and floret inoculations. Mycopathologia, 2005, 160:75-83.

13。Peschen, D., Li, H.-P., Fischer, R., Kreuzaler, F.,Liao, Y.-C^*. Fusion proteins comprising a Fusarium-specific antibody linked to anti-fungal peptides protect plants against fungal pathogens. Nature Biotechnology, 2004, 22:732-738.

14。Li, H.-P., Fu, C.-Y., Fischer, R., Liao, Y.-C^*.Cloning and characterization of a gene coding for a class I chitin synthase from Fusarium graminearum. Canadian Journal of Plant Pathology, 2003, 25:240-248.

15. Li, H.-P., Fischer, R. & Liao, Y.-C^*. Molecular evidence for induction of phenylalanine ammonia lyase during Puccinia graminis infection and elicitation in wheat. Canadian Journal of Plant Pathology, 2001, 23:286-291.