廖玉才-麦类作物分子生物技术实验室

廖玉才，博士，教授，博士生导师，麦类作物分子生物技术实验室主任，1983年获华中农业大学作物遗传育种专业硕士学位，1989年荷兰瓦赫宁根大学访问学者，1994年获德国亚琛大学分子遗传学博士学位；1995-1996年为该所博士后，1997年回国，1998-2002年到德国亚琛大学分子生物技术研究所或英国约翰英纳斯所开展合作科研。主要从事小麦与赤霉菌互作分子机理、小麦抗赤霉病遗传及其改良、镰刀菌毒素形成与控制分子机理、重组抗体分离与表达分析、植物生物反应器等研究。在《Nature Biotechnology》、《New Phytologist》、《Plant Biotechnology Journal》、《Analytical Chemistry》、《Journal of Proteome Research》、《Analytica Chimica Acta》、《Molecular Plant-Microbe Interaction》、《Fungal Genetics and Biology》、《Phytopathology》、《遗传学报》等国内外专业期刊发表论文100多篇，其中SCI刊源论文60多篇，已被引用1000多次；获省部级一等奖和三等奖各1项，指导的博士学位论文获湖北省优秀博士论文1次；获授权专利20项，申请专利7项，参编专著教材3部。现为《Journal of Phytopathology》、《Austin Biology》、《Global Journal of Biotechnology and Biomaterial Science》、《农业生物技术学报》、《麦类作物学报》和《农业科学》编委，湖北省新世纪高层次人才人选。近年来先后主持国家自然科学基金重点项目和面上项目﹑国家973和863项目﹑农业部948和转基因重大专项，以及中-欧、中-比、中-德、中-俄国际合作等多个项目。主要承担分子生物学、遗传学、植物生物技术和抗病育种教学。

发表的论文及著作（^* 通讯作者）

Selected Publications since 2003 (* corresponding author)

1. Zuo, D.-Y., Yi, S.-Y., Liu, R.-J., Qu, B., Huang, T., He, W.-J., Li, C., Li, H.-P*., Liao, Y.-C*. A deoxynivalenol-activated methionyl-tRNA synthetase gene from wheat encodes a nuclear localized protein and protects plants against Fusarium pathogens and mycotoxins. Phytopathology, 2016, DOI:10.1094/PHYTO-12-15-0327-R.

2. Song, X.-S., Xing, S., Li, H.-P., Zhang, J.-B., Qu, B., Jiang, J.-H., Fan, C., Yang, P., Liu, J.-L., Hu, Z.-Q., Xue, S., Liao, Y.-C*. An antibody that confers plant disease resistance targets a membrane-bound glyoxal oxidase in Fusarium. New Phytologist, 2015, doi: 10.1111/nph.13806.

3. Liu, N., Zhao, Z., Tan, Y., Lu, L., Wang, L., Liao, Y.-C., Beloglazova, N., De Saeger, S., Zheng, X., Wu, A*. Simultaneous raising of rabbit monoclonal antibodies to fluoroquinolones with diverse recognition functionalities via single mixture immunization. Analytical Chemistry, 2016, DOI: 10.1021/acs.analchem.5b03637.

4. Hu, Z.-Q., Li, H.-P., Liu, J.-L., Xue, S., Gong, A.-D., Zhang, J.-B*., Liao, Y.-C. Production of a phage-displayed mouse scFv antibody against Fumonisin B1 and molecular docking analysis of their interactions. Biotechnology and Bioprocess Engineering, 2016, in press.

5. Cheng, W., Song, X.-S., Li, H.-P., Cao, L.-H., Sun, K., Qiu, X.-L., Xu, Y.-B., Yang, P., Huang, T., Zhang, J.-B., Qu, B., Liao, Y.-C*. Host-induced gene silencing of an essential chitin synthase gene confers durable resistance to Fusarium head blight and seedling blight in wheat. Plant Biotechnology Journal, 2015, 13, 1335-1345.

6. Cheng, W., Li, H.-P., Zhang, J.-B., Du, H.-J., Wei, Q.-Y., Huang, T., Yang, P., Kong, X.-W., Liao, Y.-C*. Tissue-specific and pathogen-inducible expression of a fusion protein containing a Fusarium-specific antibody and a fungal chitinase protects wheat against Fusarium pathogens and mycotoxins. Plant Biotechnology Journal, 2015, 13: 664-674.

7. Li, C., Li, C.-Y., Zhang, R.-Q., Liang, W., Kang X.-L., Jia, Y., Liao, Y.-C*. Effects of drought on the morphological and physicochemical characteristics of starch granules in different elite wheat varieties. Journal of Cereal Science, 2015, 66: 66-73.

8. Gong, A,-D., Li, H.-P., Shen, L., Zhang, J.-B., Wu, A.-B., He, W.-J., Yuan, Q.-S., He, J.-D., Liao, Y.-C*. The Shewanella algae strain YM8 produces volatiles with strong inhibition activity against Aspergillus pathogens and aflatoxins. Frontiers in Microbiology, 2015, Oct 6. doi: 10.3389/fmicb.2015.01091.

9. Ma, S., Liao, Y.-C., Jevnikar, A.M. Induction of oral tolerance with transgenic plants expressing antigens for prevention/treatment of autoimmune, allergic and inflammatory diseases. Current Pharmaceutical Biotechnology, 2015, 16, 1002-1011.

10. Hu, Z.-Q., Li, H.-P., Wu, P., Li, Y.-B., Zhou, Z.-Q., Zhang, J.-B., Liu, J.-L., Liao, Y.-C*. An affinity improved single-chain antibody from phage display of a library derived from monoclonal antibodies detects fumonisins by immunoassay. Analytica Chimica Acta, 2015, 867: 74-82.

11. Gong, A.-D., Li, H.-P., Yuan, Q.-S., Song, X.-S., Yao, W., He, W.-J., Zhang, J.-B., Liao, Y.-C*. Antagonistic mechanism of iturin A and plipastatin A from Bacillus amyloliquefaciens S76-3 from wheat spikes against Fusarium graminearum. PLoS ONE, 2015 Feb 17;10(2):e0116871. doi: 10.1371/journal.pone.0116871.

12. Liu, Y.-K., Li, H.-P*., Huang, T., Gao, C.- S., Zuo, D.-Y., Zhao, Z.-X., Liao, Y.-C*. A wheat-specific gene, ribosomal protein L21, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes. Journal of Agricultural and Food Chemistry, 2014, 62: 10405-10413.

13. Wang, J.-H., J.-B. Zhang, Li, H.-P., A.-D. Gong, Liao, Y.-C*. Molecular identification, mycotoxin production and comparative pathogenicity of Fusarium temperatum isolated from maize in China. Journal of Phytopathology, 2014, 162: 147-157.

14. Song, X.-S., Li, H.-P., Zhang, J.-B., Song, B., Huang, T., Du, X.-M., Gong, A.-D., Liu, Y.-K., Feng, Y.-N. Agboola, R.S., Liao, Y.-C*. Trehalose 6-phosphate phosphatase is required for development, virulence and mycotoxin biosynthesis apart from trehalose biosynthesis in Fusarium graminearum. Fungal Genetics and Biology, 2014, 63: 24-41.

15. Wang, J.-H., Li, H.-P., J.-B. Zhang, B.-T. Wang, Liao, Y.-C*. First report of Fusarium maize ear rot caused by Fusarium kyushuense in China. Plant Disease, 2014, 98: 279-280. DOI: 10.1094/PDIS-05-13-0558-PDN.

16. Xue, S., Li, H.-P., Zhang, J.-B., Liu, J.-L., Hu, Z.-Q., Gong, A.-D., Huang, T., Liao, Y.-C*. Chicken single-chain antibody fused to alkaline phosphatase detects Aspergillus pathogens and their presence in natural samples by direct sandwich enzyme-linked immunosorbent assay. Analytical Chemistry, 2013, 85, 10992–10999.

17. Song, B., Li, H.-P., Zhang, J.-B., Wang, J.-H., Gong, A.-D., Song, X.-S., Chen T., Liao, Y.-C*. Type II myosin gene in Fusarium graminearum is required for septation, development, mycotoxin biosynthesis and pathogenicity. Fungal Genetics and Biology, 2013, 54: 60-70.

18. Saad, A. S. I., Li, X., Li, H.-P., Huang, T., Gao, C.-S., Guo, M.-W., Cheng, W., Zhao, Y.-G., Liao, Y.-C*. A rice stress-responsive NAC gene enhances tolerance of transgenic wheat to drought and salt stresses. Plant Science. 2013, 203-204: 33-40.

19. Hu, Z.-Q., Li, H.-P., Zhang, J.-B., Huang, T., Liu, J.-L., Xue, S., Wu, A.-B., Liao, Y.-C*. A phage-displayed chicken single-chain antibody fused to alkaline phosphatase detects Fusarium pathogens and their presence in cereal grains. Analytica Chimica Acta, 2013, 764: 84-92.

20. Gao, C.-S., Kou, X.-J., Li, H.-P., Zhang, J.-B., Saad, A. Sefyan I., Liao, Y.-C*. Inverse effects of Arabidopsis NPR1 gene on fusarium seedling blight and fusarium head blight in transgenic wheat. Plant Pathology, 2013, 62: 383-392.

21. Zhang, J.-B., Wang, J.-H., Gong, A.-D., Chen, F.-F., Song, B., Li, H.-P., Li, X., Peng, C.-H., Liao Y.-C*. Natural occurrence of Fusarium head blight, mycotoxins, and mycotoxin-producing strains of Fusarium in commercial fields of wheat in Hubei. Plant Pathology, 2013, 62: 92-102.

22. Han, Z., Zhao, Z. Y., Shi, J. X., Liao, Y.-C., Zhao, Z.H., Zhang, D.B., Wu, Y.L., Saeger, S.D., Wu, A.B. Combinatorial approach of LC–MS/MS and LC–TOF-MS for uncovering in vivo kinetics and biotransformation of ochratoxin A in rat. Journal of Chromatography B, 2013, 925: 46-53.

23. Liu, Z.-W., Li, H.-P., Cheng, W., Yang, P., Zhang, J.-B., Gong, A.-D., Feng, Y.-N., Fernando, W. G. D., Liao, Y.-C*. Enhanced overall resistance to Fusarium seedling blight and Fusarium head blight in transgenic wheat by co-expression of anti-fungal peptides. European Journal of Plant Pathology, 2012, 134: 721-732.

24. Hu, Z.-Q., Liu, J. L., Li, H.-P., Xing, S., Xue, S., Zhang, J.-B., Wang, J.-H., Nölke, G., Liao, Y.-C*. Generation of a highly reactive chicken-derived single-chain variable fragment against Fusarium verticillioides by phage display. International Journal of Molecular Sciences, 2012, 13, 7038-7056.

25. Ndoye, M., Zhang, J-B., Wang, J.-H., Gong, A.-D, Qu, B., Li, H.-P., Li, S.-J, Liao, Y.-C*. Nivalenol and 15-acetyldeoxynivalenol chemotypes of Fusarium graminearum clade species are prevalent on maize throughout China. Journal of Phytopathology, 2012, 160: 519-524.

26. Wang, J.-H., Zhang, J-B., Chen, F.- F., Li, H.-P., NdoyeM., Liao, Y.-C*. A multiplex PCR assay for genetic chemotyping of toxigenic Fusarium graminearum and wheat grains for 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol and nivalenol mycotoxins. Journal of Food, Agriculture and Environment. 2012, 10: 505-511.

27. Liu, J.-L., Hu, Z.-Q., Xing, S., Xue, S., Li, H.-P., Zhang, J.-B., Liao, Y.-C*. Attainment of 15-fold higher affinity of a Fusarium-specific single-chain antibody by directed molecular evolution coupled to phage display. Molecular Biotechnology, 2012, 52: 111-122.

28. Huang, T., Qu, B., Li, H.-P., Zuo, D.-Y., Zhao, Z.-X., Liao, Y.-C^*. A maize viviparous 1 gene increases seed dormancy and preharvest sprouting tolerance in transgenic wheat. Journal of Cereal Science, 2012, 55: 166-173.

29. Liu, G., Han, Z., Nie, D., Yang, J., Zhao, Z. H., Zhang, J. B., Li, H.P., Liao, Y. C., Song, S., Saeger, S. D., Wu, A. B. Rapid and sensitive quantitation of zearalenone in food and feed by lateral flow immunoassay. Food Control, 2012, 27: 200-205.

30. Han, Z., Zhao, Z.-Y., Song, S.-Q., Liu, G., Shi, J.-X., Zhang, J.-B., Liao, Y.-C., Zhang, D.-B., Saeger, S. D., Wu, A.-B. Establishment of a model isotope dilution LC-MS/MS method revealing kinetics and distribution of co-occurring mycotoxins in rat. Analytical Methods, 2012, 4, 3708–3717.

31. Chen, F. F., Zhang, J. T., Song, X.S., Yang, J., Li H. P., Tang, H^*., Liao, Y.-C^*. Combined metabonomic and quantitative real-time PCR reveal systems metabolic changes of Fusarium graminearum induced by Tri5 gene deletion. Journal of Proteome Research, 2011, 10:2273-2285.

32. Wang, J.-H., Ndoye, M., Zhang, J.-B., Li, H.-P., Liao, Y.-C*. Population structure and genetic diversity of the Fusarium graminearum species complex. Toxins, 2011, 3, 1020-1037.

33. Glinka, E. M., Liao, Y.-C. Purification and partial characterisation of pectin methylesterase produced by Fusarium asiaticum. Fungal Biology, 2011, 115: 1112-1121.

34. Amarasinghe, C., Wang, J.-H., Liao, Y.-C., Fernando, D. Difference in TRI13 gene sequences between the 3-Acetyldeoxynivalenol producing Fusarium graminearum chemotypes from Canada and China. International Journal of Molecular Science, 2011, 12, 6164-6175.

35. Li, X., Zhang, J.-B., Song, B., Li, H.-P., Xu, H. Q., Qu, B., Dang, F.-J., Liao, Y.-C^*. Resistance to Fusarium head blight and seedling blight in wheat is associated with activation of a cytochrome P450 gene. Phytopathology, 2010, 100:183-191.

36. Xu, Y.-B., Li, H.-P., Zhang, J.-B., Song, B., Chen, F.-F., Duan, X.-J., Xu, H.-Q., Liao, Y.-C^*. Disruption of the chitin synthase gene Chs1 from Fusarium asiaticum results in an altered structure of cell walls and reduced virulence. Fungal Genetics and Biology, 2010, 47: 205-215.

37. Cheng, L., Li, H.-P., Qu, B., Huang, T., Tu, J.-X., Fu, T.-D., Liao, Y.-C^*. Chloroplast transformation of rapeseed (Brassica napus) by particle bombardment of cotyledons. Plant Cell Reports, 2010, 29, 37:371-381.

38. Li, H.-P., Huang, T., Wang, C.-X., Liao, Y.-C^*. An efficient regeneration system of barley cultivars from leaf base segments. Biologia Plantarum, 2009, 53: 733-736.

39. Wang, J.-H., Li, H.-P., Qu, B., Zhang, J.-B., Huang, T., Chen, F.-F., Liao, Y.-C^*. Development of a generic PCR detection of 3-acetyldeoxynivalenol-, 15-acetyldeoxynivalenol- and nivalenol-chemotypes of Fusarium graminearum clade. International Journal of Molecular Sciences, 2008, 9:2495-2504.

40. Li, H.-P., Zhang, J.-B., Shi, R.-P., Huang, T., Fischer, R., Liao, Y.-C^*. Engineering Fusarium head blight resistance in wheat by expression of a fusion protein containing a Fusarium-specific antibody and an antifungal peptide. Molecular Plant-Microbe Interaction, 2008, 21:1242-1248.

41. Hu, Z.-Q., Li, H.-P., Zhang, J.-B., Glinka, E., Liao, Y.-C^*. Antibody-mediated prevention of Fusarium mycotoxins in the field. International Journal of Molecular Sciences, 2008, 9: 1915-1926.

42. Qu, B., Li, H.-P., Zhang, J.-B., Xu, Y.-B., Huang, T., Wu, A.B., Zhao, C.-S., Carter, J., Nicholson, P., Liao, Y.-C^*. Geographical distribution and genetic diversity of the Fusarium graminearum and F. asiaticum on wheat spikes throughout China. Plant Pathology, 2008, 57: 15-24.

43. Qu, B., Li, H.-P., Zhang, J.-B., Huang, T., Carter, J., Liao, Y.-C^*., P. Nicholson. Comparison of genetic diversity and pathogenicity of Fusarium head blight pathogens from China and Europe revealed by SSCP and seedling assays on wheat. Plant Pathology, 2008, 57:642-651.

44. Zhang, J.-B., Li, H.-P., Dang, F.-J., Qu, B., Xu, Y.-B., Zhao, C.-S., Liao, Y.-C*. Determination of the trichothecene mycotoxin chemotypes and associated geographical distribution and phylogenetic species of the Fusarium graminearum clade from China. Mycological Research, 2007, 111:967-975.

45. Liao, Y.-C*., Li, H.-P., Yao, M.-J., Zhang, J.-B., Liu, J.-L. Plantibodies: A novel strategy to create pathogen-resistant plants. Biotechnology and Genetic Engineering Reviews, 2006, 23:253-271.

46. Li, H.-P., Wu, A.-B., Zhao, C.-S., Scholten, O., Loeffler, H., Liao, Y.-C*. Development of a generic PCR detection of deoxynivalenol-and nivalenol-chemotypes of Fusarium graminearum. FEMS Microbiology Letters, 2005, 243:505-511.

47. Wu, A.-B.，Li., H.-P., Zhao, C.-S., Liao, Y.-C*. Comparative pathogenicity of Fusarium graminearum from China revealed by wheat coleoptile and floret inoculations. Mycopathologia, 2005, 160:75-83.

48. Peschen, D., Li, HP., Fischer, R., Kreuzaler, F., Liao, Y.-C*. Fusion proteins comprising a Fusarium-specific antibody linked to anti-fungal peptides protect plants against fungal pathogens. Nature Biotechnology, 2004, 22:732-738.

49. Li, H.P., Fu, CY., Fischer, R., Liao, Y.-C*. Cloning and characterization of a gene coding for a class I chitin synthase from Fusarium graminearum. Canadian Journal of Plant Pathology, 2003, 25: 240-248.