| 张静柏，女，博士，华中农业大学植物科学技术学院教师。1998-2002年就读于于河北农业大学植物保护专业，2002-2008年就读于华中农业大学攻读植物病理学专业博士学位，2008年1月获农学博士学位，毕业留校任教，承担《农业植物病理学实验》、《植物病害流行学》、《园艺植物病理学》、《园林植物病理学》等课程教学。从研究生阶段起至今，一直从事镰刀菌分子生物学、镰刀菌及其毒素特异抗体分离与毒素分析检测、镰刀菌与植物互作分子生物学、植物抗病及病菌致病相关基因克隆鉴定等方面研究，主持相关国家自然基金青年科学基金、教育部高等学校博士学科点专项新教师基金、武汉市科技攻关计划项目各1项，曾作为主要参与者完成国家“863”、“973”、“中德国际合作重点项目”多个国家课题，现作为骨干力量参加国家重大科研专项-转基因新品种培育1项、国家自然基金面上项目1项，共计发表研究论文30余篇（SCI论文32篇），授权或申请专利15项，其中以第一作者（共同第一作者）或通讯作者发表论文8篇， SCI收录6篇。 |
| 科研项目 |
1.国家自然科学基金青年科学基金项目：“赤霉菌致病相关基因CR1 的鉴定和功能分析”（2013-2015，主持）
2.教育部新教师基金：“我国长江中下游小麦、玉米赤霉菌系统群及产毒性比较研究”（2009-2012，主持）
3.武汉市科技计划项目：“抗伏马菌素特异重组抗体的分离及应用”（2013-2014，主持）
4.国家科技重大专项转基因生物新品种培育项目：“高抗赤霉病和毒素转基因小麦新品种培育”（2014-2016，参加）
5.农业部重大专项：“抗赤霉病转基因小麦新品系和新种质创制”（2011-2015，参加）
6.国家自然科学基金面上项目：“抗病抗体识别的赤霉病菌表面抗原基因克隆与功能鉴定”（2013-2016，参加） |
| 发明专利 |
授权专利
1.抗伏马菌素B1的单克隆重组抗体H2. 廖玉才，李和平，胡祖权，张静柏. 专利号号：ZL 01310211887.4.
2.抗伏马菌素B1的单克隆重组抗体H7. 廖玉才，李和平，胡祖权，张静柏. 专利号号：ZL 201310214201.7.
3.镰刀菌特异单链抗体及制备方法和应用. 廖玉才，胡祖权，李和平，张静柏.专利号：ZL 201210574749.8.
4.一种镰刀菌单端孢霉烯族A类毒素的分子鉴定方法. 廖玉才，王建华，李和平，张静柏，黄涛.专利号：ZL 201010506095.6
5.适合转基因小麦定量检测的内标准基因引物设计及应用. 廖玉才, 李和平，赵正喜，瞿波，黄涛，张静柏等. 专利号：ZL 201110432297.5.
6.玉米赤霉烯酮毒素的分子检测方法及应用. 廖玉才，王建华，李和平，张静柏等. 专利号：ZL 201010511771.9
7.一种高亲和力镰刀菌特异单链抗体及其制备方法. 廖玉才，刘锦龙，李和平，张静柏. 专利号：ZL 201110407999.8.
8.抗镰刀菌单链抗体的筛选及应用. 廖玉才，李和平，胡祖权，张静柏. 专利号：ZL 201010527093.5
9.抗伏马菌素单链抗体的筛选及应用. 廖玉才，李和平，胡祖权，张静柏. 专利号：ZL 201010527116.2
10.一种镰刀菌单端孢霉烯族B类毒素的分子鉴定方法. 廖玉才，王建华，李和平，张静柏等. 专利号：ZL 201010506091.8
11.一种小麦赤霉病穗腐和苗腐抗性的分子标记.廖玉才，李和平，李旭，瞿波，张静柏. 专利号：ZL 201010028986.5.
已申请的专利
1.一种禾谷镰刀菌葡聚糖合成酶基因GLS及应用.廖玉才，李和平，张静柏等. 申请号：201510823894.9
2.防治作物储藏期黄曲霉菌及毒素的海藻希瓦氏菌及其应用. 廖玉才，宫安东，李和平，张静柏.申请号：201510133782.0
3.一株防治小麦赤霉病的解淀粉芽孢杆菌及其应用. 廖玉才，宫安东，李和平，张静柏. 申请号：201410321676.0
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发表的论文
第一作者或共同第一作者或通讯作者文章：
1. Hu, Z.-Q., Li, H.-P., Liu, J.-L., Xue, S., Gong, A.-D., Zhang, J.-B*., Liao, Y.-C. Production of a phage-displayed mouse scFv antibody against Fumonisin B1 and molecular docking analysis of their interactions. Biotechnology and Bioprocess Engineering, 2016, 21: 134-143.
2. Wang, J.-H., Zhang, J.-B.#, Li, H.-P., Gong, A.-D., Liao, Y.-C. Molecular identification, mycotoxin production and comparative pathogenicity of Fusarium temperatum isolated from maize in China. Journal of Phytopathology, 2014, 162: 147-157.
3. Zhang, J.-B., Wang, J.-H., Gong, A.-D., Chen, F.-F., Song, B., Li, H.-P., Li, X., Peng, C.-H., Liao Y.-C*. Natural occurrence of Fusarium head blight, mycotoxins, and mycotoxin-producing strains of Fusarium in commercial fields of wheat in Hubei. Plant Pathology, 2013, 62: 92-102.
4. Li, X., Zhang, J.-B.#, Song, B., Li, H.-P., Xu, H. Q., Qu, B., Dang, F.-J., Liao, Y.-C*. Resistance to Fusarium head blight and seedling blight in wheat is associated with activation of a cytochrome P450 gene. Phytopathology, 2010, 100:183-191.
5. Li, H.-P., Zhang, J.-B.#, Shi, R.-P., Huang, T., Fischer, R., Liao, Y.-C. Engineering Fusarium head blight resistance in wheat by expression of a fusion protein containing a Fusarium-specific antibody and an antifungal peptide. Molecular Plant-Microbe Interaction, 2008, 21:1242-1248.
6. Zhang, J.-B., Li, H.-P., Dang, F.-J., Qu, B., Xu, Y.-B., Zhao, C.-S., Liao, Y.-C. Determination of the trichothecene mycotoxin chemotypes and associated geographical distribution and phylogenetic species of the Fusarium graminearum clade from China. Mycological Research, 2007, 111:967-975.
其他作者文章：
1. Song, X.-S., Xing, S., Li, H.-P., Zhang, J.-B., Qu, B., Jiang, J.-H., Fan, C., Yang, P., Liu, J.-L., Hu, Z.-Q., Xue, S., Liao, Y.-C*. An antibody that confers plant disease resistance targets a membrane-bound glyoxal oxidase in Fusarium. New Phytologist, 2015, doi: 10.1111/nph.13806.
2. Cheng, W., Song, X.-S., Li, H.-P., Cao, L.-H., Sun, K., Qiu, X.-L., Xu, Y.-B., Yang, P., Huang, T., Zhang, J.-B., Qu, B., Liao, Y.-C. Host-induced gene silencing of an essential chitin synthase gene confers durable resistance to Fusarium head blight and seedling blight in wheat. Plant Biotechnology Journal, 2015, 13, 1335-1345.
3. Cheng, W., Li, H.-P., Zhang, J.-B., Du, H.-J., Wei, Q.-Y., Huang, T., Yang, P., Kong, X.-W., Liao, Y.-C. Tissue-specific and pathogen-inducible expression of a fusion protein containing a Fusarium-specific antibody and a fungal chitinase protects wheat against Fusarium pathogens and mycotoxins. Plant Biotechnology Journal, 2015, 13: 664-674.
4. Gong, A,-D., Li, H.-P., Shen, L., Zhang, J.-B., Wu, A.-B., He, W.-J., Yuan, Q.-S., He, J.-D., Liao, Y.-C. The Shewanella algae strain YM8 produces volatiles with strong inhibition activity against Aspergillus pathogens and aflatoxins. Frontiers in Microbiology, 2015, Oct 6. doi: 10.3389/fmicb.2015.01091.
5. Hu, Z.-Q., Li, H.-P., Wu, P., Li, Y.-B., Zhou, Z.-Q., Zhang, J.-B., Liu, J.-L., Liao, Y.-C*. An affinity improved single-chain antibody from phage display of a library derived from monoclonal antibodies detects fumonisins by immunoassay. Analytica Chimica Acta, 2015, 867: 74-82.
6. Gong, A.-D., Li, H.-P., Yuan, Q.-S., Song, X.-S., Yao, W., He, W.-J., Zhang, J.-B., Liao, Y.-C. Antagonistic mechanism of iturin A and plipastatin A from Bacillus amyloliquefaciens S76-3 from wheat spikes against Fusarium graminearum. PLoS ONE, 2015 Feb 17;10(2):e0116871. doi: 10.1371/journal.pone.0116871.
7. Song, X.-S., Li, H.-P., Zhang, J.-B., Song, B., Huang, T., Du, X.-M., Gong, A.-D., Liu, Y.-K., Feng, Y.-N. Agboola, R.S., Liao, Y.-C. Trehalose 6-phosphate phosphatase is required for development, virulence and mycotoxin biosynthesis apart from trehalose biosynthesis in Fusarium graminearum. Fungal Genetics and Biology, 2014, 63: 24-41.
8. Wang, J.-H., Li, H.-P., Zhang, J.-B., Wang, B.-T., Liao, Y.-C. First report of Fusarium maize ear rot caused by Fusarium kyushuense in China. Plant Disease, 2014, 98: 279-280. DOI: 10.1094/PDIS-05-13-0558-PDN.
9. Xue, S., Li, H.-P., Zhang, J.-B., Liu, J.-L., Hu, Z.-Q., Gong, A.-D., Huang, T., Liao, Y.-C. Chicken single-chain antibody fused to alkaline phosphatase detects Aspergillus pathogens and their presence in natural samples by direct sandwich enzyme-linked immunosorbent assay. Analytical Chemistry, 2013, 85, 10992–10999.
10. Song, B., Li, H.-P., Zhang, J.-B., Wang, J.-H., Gong, A.-D., Song, X.-S., Chen T., Liao, Y.-C. Type II myosin gene in Fusarium graminearum is required for septation, development, mycotoxin biosynthesis and pathogenicity. Fungal Genetics and Biology, 2013, 54: 60-70.
11. Hu, Z.-Q., Li, H.-P., Zhang, J.-B., Huang, T., Liu, J.-L., Xue, S., Wu, A.-B., Liao, Y.-C. A phage-displayed chicken single-chain antibody fused to alkaline phosphatase detects Fusarium pathogens and their presence in cereal grains. Analytica Chimica Acta, 2013, 764: 84-92.
12. Gao, C.-S., Kou, X.-J., Li, H.-P., Zhang, J.-B., Saad, A. Sefyan I., Liao, Y.-C. Inverse effects of Arabidopsis NPR1 gene on fusarium seedling blight and fusarium head blight in transgenic wheat. Plant Pathology, 2013, 62: 383-392.
13. Liu, Z.-W., Li, H.-P., Cheng, W., Yang, P., Zhang, J.-B., Gong, A.-D., Feng, Y.-N., Fernando, W. G. D., Liao, Y.-C. Enhanced overall resistance to Fusarium seedling blight and Fusarium head blight in transgenic wheat by co-expression of anti-fungal peptides. European Journal of Plant Pathology, 2012, 134: 721-732.
14. Hu, Z.-Q., Liu, J. L., Li, H.-P., Xing, S., Xue, S., Zhang, J.-B., Wang, J.-H., Nölke, G., Liao, Y.-C. Generation of a highly reactive chicken-derived single-chain variable fragment against Fusarium verticillioides by phage display. International Journal of Molecular Sciences, 2012, 13, 7038-7056.
15. Ndoye, M., Zhang, J-B., Wang, J.-H., Gong, A.-D, Qu, B., Li, H.-P., Li, S.-J, Liao, Y.-C. Nivalenol and 15-acetyldeoxynivalenol chemotypes of Fusarium graminearum clade species are prevalent on maize throughout China. Journal of Phytopathology, 2012, 160: 519-524.
16. Wang, J.-H., Zhang, J-B., Chen, F.- F., Li, H.-P., Ndoye M., Liao, Y.-C. A multiplex PCR assay for genetic chemotyping of toxigenic Fusarium graminearum and wheat grains for 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol and nivalenol mycotoxins. Journal of Food, Agriculture and Environment. 2012, 10: 505-511.
17. Liu, J.-L., Hu, Z.-Q., Xing, S., Xue, S., Li, H.-P., Zhang, J.-B., Liao, Y.-C. Attainment of 15-fold higher affinity of a Fusarium-specific single-chain antibody by directed molecular evolution coupled to phage display. Molecular Biotechnology, 2012, 52: 111-122.
18. Liu, G., Han, Z., Nie, D., Yang, J., Zhao, Z. H., Zhang, J. B., Li, H.P., Liao, Y. C., Song, S., Saeger, S. D., Wu, A. B. Rapid and sensitive quantitation of zearalenone in food and feed by lateral flow immunoassay. Food Control, 2012, 27: 200-205.
19. Han, Z., Zhao, Z.-Y., Song, S.-Q., Liu, G., Shi, J.-X., Zhang, J.-B., Liao, Y.-C., Zhang, D.-B., Saeger, S. D., Wu, A.-B. Establishment of a model isotope dilution LC-MS/MS method revealing kinetics and distribution of co-occurring mycotoxins in rat. Analytical Methods, 2012, 4, 3708–3717.
20. Wang, J.-H., Ndoye, M., Zhang, J.-B., Li, H.-P., Liao, Y.-C. Population structure and genetic diversity of the Fusarium graminearum species complex. Toxins, 2011, 3, 1020-1037.
21. Xu, Y.-B., Li, H.-P., Zhang, J.-B., Song, B., Chen, F.-F., Duan, X.-J., Xu, H.-Q., Liao, Y.-C. Disruption of the chitin synthase gene Chs1 from Fusarium asiaticum results in an altered structure of cell walls and reduced virulence. Fungal Genetics and Biology, 2010, 47: 205-215.
22. Wang, J.-H., Li, H.-P., Qu, B., Zhang, J.-B., Huang, T., Chen, F.-F., Liao, Y.-C. Development of a generic PCR detection of 3-acetyldeoxynivalenol-, 15-acetyldeoxynivalenol- and nivalenol-chemotypes of Fusarium graminearum clade. International Journal of Molecular Sciences, 2008, 9:2495-2504.
23. Hu, Z.-Q., Li, H.-P., Zhang, J.-B., Glinka, E., Liao, Y.-C. Antibody-mediated prevention of Fusarium mycotoxins in the field. International Journal of Molecular Sciences, 2008, 9: 1915-1926.
24. Qu, B., Li, H.-P., Zhang, J.-B., Xu, Y.-B., Huang, T., Wu, A.B., Zhao, C.-S., Carter, J., Nicholson, P., Liao, Y.-C. Geographical distribution and genetic diversity of the Fusarium graminearum and F. asiaticum on wheat spikes throughout China. Plant Pathology, 2008, 57: 15-24.
25. Qu, B., Li, H.-P., Zhang, J.-B., Huang, T., Carter, J., Liao, Y.-C., P. Nicholson. Comparison of genetic diversity and pathogenicity of Fusarium head blight pathogens from China and Europe revealed by SSCP and seedling assays on wheat. Plant Pathology, 2008, 57:642-651.
26. Liao, Y.-C., Li, H.-P., Yao, M.-J., Zhang, J.-B., Liu, J.-L. Plantibodies: A novel strategy to create pathogen-resistant plants. Biotechnology and Genetic Engineering Reviews, 2006, 23:253-271.
中文文章：
1. 胡祖权，李和平，刘锦龙，吴平，廖玉才，张静柏*. 抗伏马菌素B1单克隆重组抗体的筛选. 基因组学与应用生物学，2015，34(9)：1912-1916
2. 胡祖权，李和平，吴平，廖玉才，张静柏*. 抗镰刀菌单链抗体在大肠杆菌中可溶性表达条件的研究. 生物技术通报，2015，31(9)：238-243
3. 胡祖权，李和平，张静柏，刘锦龙，廖玉才. 伏马菌素B1特异单链抗体的同源建模及分子对接模拟研究. 免疫学杂志，2015，31(7)：618-622
4. 李和平，张静柏，廖玉才. 绿色荧光蛋白基因在小麦禾谷镰刀菌中的表达与鉴定. 麦类作物学报，2010，30(5)： 824-828
5. 刘春雷，廖玉才，张静柏，黄涛，李和平. 赤霉病菌特异抗体-防御素融合蛋白的表达和功能鉴定. 华中农业大学学报，2007，26(4)：435-438
6. 武爱波，李和平，张静柏，赵纯森，姚明镜，SCHOLTEN Olga，廖玉才. 中国与欧洲禾谷镰刀菌DON毒素HPLC定量比较分析. 应用与环境生物学报，2007，13(1)：131-134
7. 武爱波，李和平，张静柏，姚明镜，许玉彬，廖玉才. 小麦和玉米籽粒赤霉菌产毒类型的PCR检测. 麦类作物学报，2005，25( 5)：1-4 |
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